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LAP Disc Test
The LAP Disc Test is used to detect the enzyme leucine aminopeptidase (LAP), one of the tests considered as a definitive identification test for catalase negative gram positive cocci (1,2). Specifically, it is used to differentiate Aerococcus and Leuconostoc from Streptococcus, Enterococcus, Lactococcus and Pediococcus.
The LAP Disc Test is used to detect the enzyme leucine aminopeptidase, one of the tests considered as a definitive identification test for catalase negative gram positive cocci. (1,2)
Aerococcus and Leuconostoc both do not contain LAP and will yield negative results. Streptococcus, Enterococcus, Lactococcus and Pedicoccus are LAP positive. Aerococcus and Leuconostoc can be easily differentiated by the PYR and other tests. (2)
Hydrolysis of leucine p-Naphthylamide releases pure naphthylamide which is red after adding the PEP Reagent.
(1) Colman, G. and L.C. Ball, 1984. Identification of Streptococci in the Medical Laboratory. J. appl. Microbiol. 57:1-14.
(2) Facklam, R.R. and J.A. Washington, 1990. Streptococci and Related Catalase Negative Gram-positive Cocci. Manual of Clinical Microbiology, 5th Ed. ASM. Washington, D.C.
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