Adeno-Rota Virus Combo Test Card
The Adeno-Rota Combo Test is an in vitro qualitative immunochromatographic assay for the rapid detection of adenovirus and rotavirus antigens in human stool specimen. The test results are intended to aid in the diagnosis of adenovirus and rotavirus infection and to monitor the effectiveness of therapeutic treatment. This product is intended for Laboratory use only.
Rotavirus is the primary causative agent of pediatric gastroenteritis and diarrhea worldwide. Almost every child on the planet may get infected by age 5. Scientists say that 900,000 young children around the world die each year from rotaviruses. In tropical climates, rotavirus infection can occur all year round. The age groups most susceptible to the disease are that of infants and children. The infection usually begins with a fever. Soon the little one begins to vomit and has a nasty stomachache. The vomiting goes away, followed by watery diarrhea that lasts from 3 to 9 days. Most of the time, kids recover easily. Sometimes, severe dehydration will result. The infection starts suddenly and lasts for an average of four to six days. Rotaviruses are extremely contagious. Only a few particles are needed to transmit infection. They originate in the stool but are found throughout the environment wherever young children spend much time.
They are resistant to disinfectants used to clean surfaces and to anti-bacterial hand-washing agents. Rotavirus particles remain active on human hands for at least 4 hours, on hard dry surfaces for 10 days, and on wet areas for weeks. Rotavirus induced dehydration is a major cause of infant morbidity in both developed and underdeveloped countries, and a major cause of infant mortality in the developing countries. Adenovirus is the second most common cause of viral gastro-enteritis in Children (10- 15%).
This virus may also cause respiratory diseases and, depending on the serotype, also diarrhea, conjunctivitis, cystitis, etc. At least 47 serotypes of adenovirus have been described, all sharing a common hexon antigen. Serotypes 40 and 41 are the ones associated with gastro-enteritis.
The Adeno-Rota Combo Test is a sandwich solid phase immunochromatographic assay. To perform the test, an aliquot of diluted stool sample is added to the sample well of the test cassette. The sample flows through a pad containing antibodies against adenovirus and rotavirus coupled to red-colored colloidal gold. If the sample contains adenovirus or rotavirus antigens, the antigen will bind to the antibody coated on the colloidal gold particles to form antigen-antibody-gold complexes. These complexes move on the nitrocellulose membrane by capillary action toward the test line region on which adenovirus and rotavirus specific antibodies are immobilized separately. As the complexes reach the test line, they will bind to the antibody corresponding to the virus on the membrane to form of a line. A red control line will always appear in the result window to indicate that the test has been correctly performed and the test device functions properly. If virus is not present or lower than the detection limit of the test, only the control line will be visible. If the control line does not develop, the test is invalid.
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