Systemic Lupus Erythematosus

This test is used for diagnosing Systemic Lupus Erythematosus and detects and quantifies the serum antinuclear antibodies (ANAs). 

 

Antinuclear antibodies are autoantibodies that are mistakenly antibodies to human antigens and bind to the cell nucleus. Methods to detect antinuclear antibodies (ANAs) in the laboratory include immunofluorescence, the Lupus Erythematosus (LE) cell test and agglutination of coated particles. The Systemic Lupus Erythematosus test uses the method of agglutination. The ANAs that are the most characteristic of Systemic Lupus Erythematosus are directed against deoxyribonucleoprotein (DNP). These antibodies cause the formation of the LE cell in vitro. 

 

The principle of the Systemic Lupus Erythematosus test is based on the agglutination reaction between latex particles coated with DNP and a serum which contains ANAs (this is an antigen/antibody reaction). An agglutination will take place within a minute and indicates a positive reaction. 

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